ABSTRACT
Group B streptococcus is the main reason of neonatal infection in developed countries and causes a widespread clinical indications. In developing countries such as Iran, its rate of appearance is not determined. With regard to colonization and the relative high epithelia of group B streptococcus, it is likely that the incidence of group B streptococcus in neonatal sepsis is so high. In the present study, we attempted to use the molecular methods to identify this bacterium and develop the culture environments as well. In the present study, a hundred below three months year old infants with sepsis hospitalized in ICU sector of Ghaem hospital for one year were studied since Khordad 1388 [June 2010]. After getting consent from the infants' parents, three blood samples of these patients in the sterile container with lid were transferred to the laboratory [two samples for culturing in normal environment and the other for PRC]. All of the information was filled out by doctors in the incidence. SPSS 11.5 and descriptive-analytic tests were used for data analysis. 100 infants less than three month, with clinical signs of sepsis were enrolled. Group B Streptococcus and did not grow in none of the culture three infants had positive PCR for Group B Streptococcus, 5 cases had positive normal and developed culture and 6 cases had negative normal and developed culture. 64 percent of pregnant women have taken antibodies before delivery. With regard to the high rate of anti-biotic consumption by mothers before delivery, it is necessary to use more sensitive methods like PCR to identify the Group B Streptococcus